醫用純Mg表面多種復合處理膜層的組織結構和體內外性能

醫用純Mg表面多種復合處理膜層的組織結構和體內外性能The Microstructure and in Vivo and Vitro Property of multi-component composite Films on the Biomedical Pure Magnesium Alloy Surface

為了提高醫用純Mg的耐蝕性和生物活性，采用超聲微弧氧化（UMAO）、堿處理（OH）、硅烷轉化膜處理（SCA）和自組裝絲素蛋白（SF）多種處理方法，在Mg表面形成多元膜層（UMAO-OH-SCA-SF），通過SEM、紅外光譜、電化學測試、細胞實驗、動物植入實驗，研究膜層表面形貌、組織結構、耐腐蝕性能、細胞活性和體內骨生長。結果表明，超聲微弧氧化膜層以MgO為主相，堿羥基利于與硅烷偶聯形成Si-O-Mg膜，再自組裝絲素，隨著浸泡絲素蛋白時間增加，絲素蛋白的結構由無規卷曲向β-折疊轉變，多元復合膜層提高自腐蝕電位，自腐蝕電流密度降低了兩個數量級。多元復合膜層更能促進成骨細胞的增殖、黏附與分化，具有良好的早期骨整合能力和在純Mg降解過程中控制Mg2+溶出能力。其中UMAO-OH-SCA-SF/1.5h最佳。

Entering into 21 Century，the degradable Mg and Mg alloy become research focus for the development of Internal fixation material from inert to active metal. The poly-basic coating (UMAO-OH-SCA-SF) was prepared by ultrasound micro oxidation (UMAO), alkali treatment (OH), treatment of silicohydride conversion coating (SCA) and the treatment of self-assembly silk fibroin on the surface of magnesium, which can improve the corrosion resistance and bioactivity of pure magnesium. The surface topography, structure, corrosion resistance, cell activity and bone growth in vivo of the coating were studied by scanning electron microscopy (SEM), IR spectra, electrochemical measurement, vitro experiment and implant test. The results show that: the main phase of coating is MgO. The alkali treatment is beneficial to form Si-O-Mg film by silicohydride coupling. With the self-assembly silk fibroin time increasing, the silk fibroin structure changes from random coil to β-fold. The poly-basic coating self-corrosion is improved and self-corrosion current is reduced by two orders of magnitude. Compared to substrate, the poly-basic coating has better proliferation, adherent and differentiation of osteoblast . It has a better bone integration capacity in the bone healing early stage, and which can control magnesium ion dissolving. The UMAO-OH-SCA-SF/1.5h coating has the best property.

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